Journal article
Cellular mechanisms underlying temporal changes in skeletal muscle protein synthesis and breakdown during chronic β-adrenoceptor stimulation in mice
R Koopman, SM Gehrig, B Léger, J Trieu, S Walrand, KT Murphy, GS Lynch
Journal of Physiology | Published : 2010
Abstract
Chronic stimulation of β-adrenoceptors with β-adrenoceptor agonists (β-agonists) can induce substantial skeletal muscle hypertrophy, but the mechanisms mediating this muscle growth have yet to be elucidated. We investigated whether chronic β-adrenoceptor stimulation in mice with the β-agonist formoterol alters the muscle anabolic response following β-adrenoceptor stimulation. Twelve-week-old C57BL/6 mice were treated for up to 28 days with a once-daily injection of either saline (control, n= 9) or formoterol (100 μg kg -1; n= 9). Rates of muscle protein synthesis were assessed at either 1, 7 or 28 days of treatment, 6 h after injection. Protein synthesis rates were higher in formoterol-treat..
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Awarded by National Health & Medical Research Council (NHMRC, Australia)
Funding Acknowledgements
This work was supported by grants from the National Health & Medical Research Council (NHMRC, Australia, Project grant 509313) and the Association Francaise contre les Myopathies (France). R.K. is supported by a C. R. Roper Research Fellowship from the Faculty of Medicine, Dentistry and Health Sciences of The University of Melbourne. B.L. is supported by a Fellowship from the Swiss National Funds. K.T.M. is supported by a Biomedical Australian Fellowship from the National Health and Medical Research Council (NHMRC). S.M.G. was supported by a Postgraduate Scholarship from the National Heart Foundation (Australia). We thank Benjamin Gleeson for expert technical assistance.